Ni-NTA sepharose Purification kit
Affinity purified Ni-NTA is conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or other proteins.
The Ni-NTA sepharose is specifically designed for the purification of recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, insects, and mammalian cells. The sepharose is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.
The Ni-NTA resin can be used to purify 6 x His tagged proteins under native and denaturing conditions. Proteins bound to the resin can be eluted with low pH buffer or competition with imidazole or histidine.
The Ni-NTA sepharose is specifically designed for the purification of recombinant proteins fused to the 6 x histidine (6 x His) tag expressed in bacteria, insects, and mammalian cells. The sepharose is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.
The Ni-NTA resin can be used to purify 6 x His tagged proteins under native and denaturing conditions. Proteins bound to the resin can be eluted with low pH buffer or competition with imidazole or histidine.
Component:
Note: Sepharose is 1:1 suspension in Phosphate Buffered Saline, pH 7.4, containing 0.05% sodium azide as a preservative.
Product capacity:
The binding and elution capacity of 1 mL settled Ni-NTA sepharose are commonly more than 5 mg of 6 x His fusion protein. Trying different wash buffers and elution buffers for optimal results is recommended.
Materials Required but Not Provided:
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice
| Reagent | Quantity for 10 rxns (C07008-K02) |
Quantity for 5 rxns (C07008-K01) |
composition |
| Ni-NTA sepharose |
2 mL X 1 vial | 1 mL X 1 vial | 50% slurry of Ni-NTA sepharose in 1X Phosphate Buffered Saline |
| Loading Buffer | 50 mL X 1 vial | 25 mL X 1 vial | 20 mM sodium phosphate; 500 mM NaCl, pH 7.4 |
| Wash Buffer | 50 mL X 1 vial | 25 mL X 1 vial | 20 mM sodium phosphate; 500 mM NaCl; 20 mM imidazole, pH 7.4 |
| Elution Buffer | 10 mL X 1 vial | 5 mL X 1 vial | 20 mM sodium phosphate; 500 mM NaCl; 500 mM imidazole, pH 7.4 |
| spin column | 10 pcs | 5 pcs | |
| collection tube | 10 tubes | 5 tubes |
Product capacity:
The binding and elution capacity of 1 mL settled Ni-NTA sepharose are commonly more than 5 mg of 6 x His fusion protein. Trying different wash buffers and elution buffers for optimal results is recommended.
Materials Required but Not Provided:
- Micro-centrifuge capable of 15,000 x g
- 1.5 mL Centrifuge tubes
- End-over-end rotator
- CoIP Lysis Buffer (mild reaction): 10 mM Tris/Cl pH 7.5; 150 mM NaCl; 0.5 mM EDTA; 0.5% NP-40
- RIPA (vigorous reaction): 100 mM Tris/Cl pH 7.5; 300 mM NaCl; 0.2% Sodium Deoxycholate (or 0.1% SDS); 2% NP-40
For sustainable use and long term storage, store at 2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice